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| From
the Editor |
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Editorial
A. Abyad (Chief Editor) |
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Original contribution/Clinical Investigation
Diabetes Mellitus
- Knowledge, Management and Complications: Survey
report from Faisalabad-Pakistan
Ijaz Anwer, Ahmad Shahzad, Kashmira Nanji, Farah
Haider, Muhammad Masood Ahmad
Alanine aminotransferase
indicates excess weight and dyslipidemia
Mehmet Rami Helvaci, Orhan Ayyildiz* Mustafa
Cem Algin, Yusuf Aydin, Abdulrazak Abyad, Lesley
Pocock
Comparative Analysis of Antimicrobial Peptides
Gene Expression in Susceptible/Resistant Mice
Macrophages to Leishmania major Infection
Hamid Daneshvar, Iraj Sharifi, Alireza Kyhani,
Amir Tavakoli Kareshk, Arash Asadi
Does
socio-economic status of the patients have effect
on clinical outcomes after coronary artery bypass
grafting surgery?
Forough Razmjooei, Afshin Mansourian, Saeed
Kouhpyma
Comparison of the uterine
artery Doppler indices during pregnancy between
gestational diabetes and diabetes mellitus and
healthy pregnant women
Nazanin Farshchian, Farhad Naleini, Amir Masoud
Jaafarnejhad,
Parisa Bahrami Kamangar
Survey single dose
Gentamicin in treatment of UTI in children with
range of 1 month to 13 years old in Jahrom during
2015
Ehsan Rahmanian, Farideh Mogharab,
Vahid Mogharab
Evaluation of control of
bleeding by electro cauterization of bleeding
points of amplatz sheath tract after percutaneous
nephrolithotomy (PCNL) in Jahrom Peymanieh hospital
during year 2015-2016
Ali Reza Yousefi , Reza Inaloo
Comparison of the three-finger
tracheal palpation technique with triple ID
formula to determine endotracheal tube depth
in children 2-8 years in 2016-2017
Anahid Maleki, Alireza Ebrahim Soltani, Alireza
Takzare, Ebrahim Espahbodi,
Mehrdad Goodarzi , Roya Noori
Effect of Sevoflurane
and Propofol on pulmonary arterial pressure
during cardiac catheterization in children with
congenital heart diseases
Faranak Behnaz, Mahshid Ghasemi , Gholamreza
Mohseni, Azim Zaraki
Population and Community Studies
Prevalence
and risk factors of obesity in children aged
2-12 years in the Abu Dhabi Islands
Eideh Al-Shehhi, Hessa Al-Dhefairi, Kholoud
Abuasi, Noora Al Ali, Mona Al Tunaiji, Ebtihal
Darwish
Study and comparison
of psychological disorders in normal students
and students with multiple sclerosis in Shahrekord
Neda Ardestani-Samani, Mohammad Rabiei, Mohammad
Ghasemi-Pirbalooti, Asghar Bayati, Saeid Heidari-Soureshjani
Comparative
study of self-concept, physical self-concept,
and time perspective between the students with
multiple sclerosis and healthy students in Shahrekord
Neda Ardestani-Samani, Mohammad Rabiei, Mohammad
Ghasemi-Pirbalooti, Asghar Bayati, Saeid Heidari-Soureshjani
Relationship
between Coping Styles and Religious Orientation
with Mental Health in the Students of the Nursing-Midwifery
Faculty of Zabol
Nasim Dastras, Mohsen Heidari Mokarrar, Majid
Dastras, Shirzad Arianmehr
Tuberculosis in Abadan,
Iran (2012-2016): An Epidemiological Study
Ali-Asghar ValiPour, Azimeh Karimyan, Mahmood
Banarimehr, Marzieh Ghassemi, Maryam Robeyhavi,
Rahil Hojjati,
Parvin Gholizadeh
Family Stability and Conflict
of Spiritual Beliefs and Superstitions among
Yazdi People in Iran: A Qualitative Study
Zahra Pourmovahed , Seyed Saied Mazloomy Mahmoodabad
; Hassan Zareei Mahmoodabadi ; Hossein Tavangar
; Seyed Mojtaba Yassini Ardekani ; Ali Akbar
Vaezi
A comparative
study of the self-actualization in psychology
and Islam
Simin Afrasibi, Zakieh Fattahi
The effectiveness
of cognitive - behavioral therapy in reducing
the post-traumatic stress symptoms in male students
survivors of earthquake in the central district
of Varzeghan
Sakineh Salamat, Dr.Ahad Ahangar, Robab Farajzadeh
Reviews
Effects and mechanisms
of medicinal plants on stress hormone (cortisol):
A systematic review
Kamal Solati, Saeid Heidari-Soureshjani, Lesley
Pocock
Comparing Traditional
and medical treatments for constipation : A
Review Article
Mohammad Yaqub Rajput
A review of anti-measles
and anti-rubella antibodies in 15- 25 year old
women in Jahrom City in 2011
Ehsan Rahmania , Farideh Mogharab, Vahid Mogharab
Review of percutaneous
nephrolithotomy in children below 12 years old
in Jahrom hospital, during 2010-2014
Ali Reza Yousefi , Reza Inaloo
Physical and
mental health in Islam
Bahador Mehraki, Abdollah Gholami
International Health Affairs
The Challenges of Implementation
of Professional Ethics Standards in Clinical Care
from the viewpoint of Nursing Students and Nurses
Saeedeh Elhami, Kambiz Saberi, Maryam Ban, Sajedeh
Mousaviasl, Nasim Hatefi Moadab, Marzieh Ghassemi
Cognitive Determinants
of Physical Activity Intention among Iranian Nurses:
An Application of Integrative Model of Behavior
Prediction
Arsalan Ghaderi, Firoozeh Mostafavi, Behzad Mahaki,
Abdorrahim Afkhamzadeh,
Yadolah Zarezadeh , Erfan Sadeghi
Effect of resilience-based
intervention on occupational stress among nurses
Hossein Jafarizadeh, Ebrahim Zhiyani, Nader
Aghakhani, Vahid Alinejad, Yaser Moradi
Education and Training
Calculation of Salaries
and Benefits of Faculty Members in the Ministry
of Health and Medical Education of Iran
Abdolreza Gilavand
The effect of education
on self-care behaviors of gastrointestinal side
effects on patients undergoing chemotherapy
Shokoh Varaei, Ehsan Abadi Pishe, Shadan Pedram
Razie, Lila Nezam Abadi Farahani
Creating and
Validating the Faith Inventory for Students
at Islamic Azad University of Ahvaz
Solmaz Choheili, Reza Pasha, Gholam Hossein
Maktabi, Ehsan Moheb
Creating
and Validating the Adjustment Inventory for
the Students of Islamic Azad University of Ahvaz
Homa Choheili, Reza Pasha, Gholam Hossein Maktabi,
Ehsan Moheb
Evaluating
the Quality of Educational Services from the
Viewpoints of Radiology Students of Ahvaz Jundishapur
University of Medical Sciences
Abdolreza Gilavand, Jafar Fatahiasl
An Investigation
of Psychosocial aspect of Iranian Nursing Students'
Clinical Setting
Mahsa Boozaripour , Zanyar Karimi, Sima Zohari
Anbohi, Amir Almasi-Hashiani, Fariba Borhani
Clinical Research and Methods
Comparison of the
Antibacterial Effects of Chlorhexidine Mouth
washes with Jaftex Mouth wash on Some Common
Oral Microorganisms (An in Vitro Study)
Ebrahim Babadi, Zahra Bamzadeh, Fatemeh Babadi
Study of the effect
of plasma jet on Fusarium isolates with ability
to produce DON toxins
Elham Galin Abbasian, Mansour Bayat, Arash chaichi
Nosrati, Seyed Jamal Hashemi, Mahmood Ghoranneviss
The comparison of
anti-inflammatory effect in two methods of topical
dexamethasone injection and topical application
of ginger alcoholic extract after removing mandibular
wisdom teeth
Sahar Zandi, Seyyed Muhammadreza Alavi, Kamran
Mirzaie, Ramin Seyedian, Narges Aria, Saman
Jokar
The effect of curcumin
on growth and adherence of major microorganisms
causing tooth decay
Leila Helalat, Ahmad Zarejavid, Alireza Ekrami,
Mohammd Hosein Haghighizadeh, Mehdi Shiri Nasab
|
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Chief
Editor -
Abdulrazak
Abyad
MD, MPH, MBA, AGSF, AFCHSE
.........................................................
Editorial
Office -
Abyad Medical Center & Middle East Longevity
Institute
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PO BOX 618
Tripoli, Lebanon
Phone: (961) 6-443684
Fax: (961) 6-443685
Email:
aabyad@cyberia.net.lb
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Pocock
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| November 2017
- Volume 15, Issue 9 |
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|
Study of the effect of
plasma jet on Fusarium isolates with ability
to produce DON toxins
Elham Galin Abbasian (1)
Mansour Bayat (1)
Arash Chaichi Nosrati (2)
Seyed Jamal Hashemi (3)
Mahmood Ghoranneviss (4)
(1) Department
of Microbiology, Science and Research Branch,
Islamic Azad University,Tehran, Iran
(2) Associate Professor, Division microbiology,
Department of molecular & cell biology,
Faculty of basic sciences, Lahijan branch, Islamic
Azad university (IAU), Lahijan, Gilan, Iran
(3) Professor of Tehran University of medical
Sciences, Tehran, Iran
(4) Professor of physics, Plasma physics research
center, Science and Research Branch, Islamic
Azad University, Tehran, Iran
Corresponding author:
Mansour
Bayat
Professor of Physics, Plasma physics research
center,
Science and Research Branch, Islamic Azad University,
Tehran, Iran
Email:
dr_mansour_bayat@yahoo.com
|
Abstract
Introduction:
Fusarium species cause a variety of infections
in humans, such as superficial, invasive
and disseminated infections. The aim of
this research is to study the effects
of argon cold double atmospheric pressure
plasma (DAPACP) on deactivating the cells
or Fusarium spores which can seriously
damage food products in vitro conditions.
Materials and
Methods: Samples were obtained from
May 2015 to September 2016 during three
cultivation periods of tea in Gilan and
Mazandaran province. One sample was taken
from each 50 square hectares of tea plantations
prepared for harvesting and also from
60 tea manufacturers. At intervals of
3, 7 and 15 days, plates were studied
and the colonies on each plate were identified
and isolated and then their microscopic
and also macroscopic properties were documented.
Finally 150 colonies were selected. We
prepared Czapek and liquid environments
(Czpk), then the isolated samples were
cultured using stab. Plasma system is
a DAPACP system (AL2O3) which used a high
voltage with 25KHZ frequency and is usually
applied to a high voltage electrode.
Results: The
amount and type of toxins in the environment
with fixed toxicity vary . In the metabolic
cycle, DON (deoxynivalenol) is first produced
and then is metabolized in a few steps.
Plasma jet treatment causes a relative
reduction in concentration of DON per
solvent volume or culture media; a statistically
significant negative correlation is observed
here. The negative correlation between
DON, which has been measured in samples
previously exposed to plasma jet for 60
seconds , is affected by plasma jet more
than DON. Therefore, Z = -2 / 201, Sig
= 0.028 is proven, and it occurs with
more intensity compared to DON.
Conclusion:
This study has confirmed that argon
plasma jet system has destructive effects
on mycotoxins such as DON and also on
the microorganisms which produce mycotoxins.
Although the physicochemical properties
and structural changes of mycotoxins were
not explained under plasma jet treatment,
this study revealed that the plasma system
needs certain conditions like time and
toxin concentration of food, to remove
and inactivate mycotoxins.
Key words:
Fusarium, plasma jet, DON, toxins
|
Fusarium species cause a variety of infections
in humans, such as superficial, invasive and
disseminated infections [1]. Clinical manifestation
of Fusariosis depends on the host immune system
and the entrance site of the organism [2]. Superficial
and localized infections occur mainly in people
with healthy immune systems, while invasive
and disseminated infections have been seen in
immunocompromised persons [3]. Fusarium species
are considered as pathogenic agents in plants,
crops and in some animals [4]. Fusarium species
are widely distributed in soil and water [5,
6]. More than 50 species of Fusarium have been
identified, twelve of which are associated with
disease including; Fusariumsolani, Fusarium
oxysporum and Fusarium which contributes to
nearly 70 % of the cases [7, 8].
Fusarium species carry several virulence factors,
including Mycotoxins which are cellular and
humoral immune suppressive and cause tissue
problems [9]. In addition, Fusarium species
have the ability to combine to prosthetic materials
and could also produce protease and collagenase
[10].
Deoxynivalenol (DON) is one of the several
Mycotoxins produced by Fusarium species which
causes contamination in maize, wheat, barley,
rice and other grains during their storage life
and it is considered a risk factor for humans
and animals [11]. This toxin causes nausea or
vomiting and was first reported in Japan in
1972 following the consumption of mouldy barley
[12].
DON is chemically a member of trichothecenes
mycotoxins family and structurally is apolar
organic material belonging to trichothecenes
type B, with three free hydroxyl groups which
causes its toxicity. One of the most significant
physicochemical properties of DON is high-temperature
heat resistance and water solubility [13].
Plasma as a state of matter has significantly
different characteristics; plasma has no fixed
shape or volume and can form magnetic lines
and rays under magnetic fields. In accordance
to this method used, plasma can be converted
into a wide range of states; from being unbalanced
to thermal equilibrium. The antimicrobial effects
of plasma have been known for almost 50 years
[18, 16].
Research has mainly focused on the use of plasma
on nonliving surfaces, such as medical instruments.
Plasma is a cold gas-like mixture of charged
particles (free electron and proton particles)
with neutral reactive particles including; gas
molecules, free radicals and ultraviolet photons.
When the reactor is launched in atmospheric
pressure, a cool plasma jet is blown into the
air [17]. Since this plasma jet can be used
in sterilization of sensitive surfaces, it is
a proper candidate to be applied instead/with
the autoclave sterilization process for surgical
and dental use as an effective agent to destroy
bacteria and viruses [15].
The aim of this research is to study the effects
of argon cold double atmospheric pressure plasma
(DAPACP) on deactivating the cells of Fusarium
spores which can seriously damage food products
in vitro conditions. Moreover, fungi are used
to investigate the efficacy of cold plasma in
suppressing the produced mycotoxins.
Sampling:
Samples
were
obtained
from
May
2015
to
September
2016
during
three
cultivation
periods
of
tea
in
Gilan
and
Mazandaran
province
through
following
the
instructions
of
outdoor
and
indoor
sampling.
One
sample
was
taken
from
each
50
square
hectares
of
tea
plantations
that
were
prepared
for
harvesting
and
also
from
60
tea
manufacturers.
Sampling
was
carried
out
by
placing
open
plates
at
a
height
of
90-110
cm,
3
to
5
days
after
each
rainfall
from
9
o’clock
till
3
pm
during
sunshine
at
25
±
3
°
C
and
when
their
velocity
was
measured
at
30
m
/
s.
Six
plates
with
malt
extract
agar,
yeast
extract
agar,
Czapek
yeast
extract
agar,
Czapek
agar,
sabouraud
dextrose
agar
and
potato
dextrose
agar
were
all
mixed
with
100
ppm
chloramphenicol
and
50
ppm
tetracycline
and
were
examined
3
to
5
days
at
25
°
C
before
operation.
All
of
them
were
applied
for
one
group
of
samples.
Plates
containing
15
to
25
cubic
centimeter
of
agar
after
30,
60
and
90
minutes
were
sampled
through
plantations
and
15,
30
and
60
minutes
through
factories;
900
plates
were
taken
and
then
were
placed
in
perforated
polyethylene
bags
and
sent
to
the
laboratory.
The
plates
were
incubated
at
25
±
2
°
C
in
an
aerobic
environment.
One
plate
was
kept
in
the
dark,
another
one
in
the
light
and
finally
a
pair
of
them
was
kept
in
light-dark
enviroment.
At
intervals
of
3,
7
and
15
days,
plates
were
studied
and
the
colonies
on
each
plate
were
detected
and
isolated
and
then
their
microscopic
and
macroscopic
properties
were
documented.
Finally150
colonies
were
selected.
Cultivation
&
Isolation:
We
prepared
Czapekand
liquid
environments
(Czpk),
then
the
isolated
samples
were
cultured
using
stab
(in
3
places
with
a
distance
of
2
cm
from
each
other
and
from
the
edge
of
the
plate).
Then
they
were
incubated
for
14
days
at
25
°
C.
Samples
were
then
cultured
in
liquid
environments
of
sB
+
ME
and
sB
+
yE.
They
were
checked
regularly
to
avoid
drying
and
reduction
in
the
volume
of
liquid,
PBS
was
added
to
these
environments.
The
samples
were
collected,
and
the
extracted
solution
was
added
and
filtered.The
slides
of
the
cultures
were
prepared
and
then
stained
with
lacto
phenol.
Extracted
solutions
from
solid
and
liquid
environments
were
prepared
for
ELISA
technique.
Then
the
samples
were
exposed
to
cold
plasma
jet
and
were
set
at
30
seconds,
60
seconds
and
360
seconds
in
certain
concentrations.
At
this
stage,
sample
volume
was
reduced.
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